To calculate the final concentration of a primer after dilution:
\[ \text{FC} = \frac{\text{IC} \times \text{V1}}{\text{V2}} \]
Where:
A primer dilution is a laboratory procedure used in molecular biology to reduce the concentration of a primer solution. Primers are short strands of DNA or RNA that serve as a starting point for DNA synthesis. They are often supplied in high concentrations, which can be too strong for certain experiments. Therefore, they need to be diluted to a suitable concentration. This is done by adding a specific amount of solvent to the primer solution, typically using distilled water or a buffer solution. The resulting diluted primer solution can then be used in various molecular biology techniques such as polymerase chain reaction (PCR) or DNA sequencing.
Let's assume the following values:
Using the formula:
\[ \text{FC} = \frac{100 \times 50}{500} = 10 \, \text{µM} \]
The Final Concentration is 10 µM.
Let's assume the following values:
Using the formula:
\[ \text{FC} = \frac{200 \times 100}{1000} = 20 \, \text{µM} \]
The Final Concentration is 20 µM.
