Concentration from Absorbance Calculator

Formula

The formula to calculate the concentration from absorbance is:

\[ C = \frac{A}{L \times e} \]

Where:

\( C \) is the concentration
\( A \) is the absorbance
\( L \) is the path length (cm)
\( e \) is the extinction coefficient (L/(mol*cm))

What is Concentration?

Concentration is defined as the total amount of a substance in a given space. It is a crucial parameter in chemistry and biology to quantify the amount of solute in a solution.

Example Calculation

Let's assume the following values:

Absorbance (A): 0.5
Path Length (L): 1 cm
Extinction Coefficient (e): 100 L/(mol*cm)

Using the formula to calculate the concentration:

\[ C = \frac{0.5}{1 \times 100} = 0.005 \text{ mol/L} \]

The concentration is 0.005 mol/L.