The formula to calculate the qPCR Efficiency is:
\[ E = -1 + 10^{-\frac{1}{S}} \]
Where:
Let's say the slope (S) is -3.32. The qPCR efficiency would be calculated as follows:
\[ E = -1 + 10^{-\frac{1}{-3.32}} \approx 1.0008 \text{ or } 100\% \]
So, the qPCR efficiency is approximately 100.08%.
qPCR efficiency is a measure of how effectively a quantitative PCR (qPCR) amplifies the target DNA. It is calculated based on the slope of the standard curve generated during the qPCR experiment. An efficiency of 100% indicates that the amount of DNA doubles with each cycle.
