Primer Efficiency Calculator



Formula

To calculate the Primer Efficiency (E):

\[ E = 10^{\left(-\frac{1}{\Delta Ct}\right)} - 1 \]

Where:

What is Primer Efficiency?

Primer efficiency is a measure of how effectively a primer set amplifies a target DNA sequence in a polymerase chain reaction (PCR). It is an important parameter in quantitative PCR (qPCR) as it affects the accuracy of the quantification. High primer efficiency indicates that the primers are well-designed and that the PCR conditions are optimal for the amplification of the target sequence.

Example Calculation 1

Let's assume the following value:

Step 1: Use the formula:

\[ E = 10^{\left(-\frac{1}{3.32}\right)} - 1 = 10^{-0.301} - 1 = 0.5 - 1 = -0.5 \times 100 = -50\% \]

The Primer Efficiency is -50%.

Example Calculation 2

Let's assume the following value:

Step 1: Use the formula:

\[ E = 10^{\left(-\frac{1}{3.0}\right)} - 1 = 10^{-0.333} - 1 = 0.464 - 1 = -0.536 \times 100 = -53.6\% \]

The Primer Efficiency is -53.6%.