To calculate the Primer Efficiency (E):
\[ E = 10^{\left(-\frac{1}{\Delta Ct}\right)} - 1 \]
Where:
Primer efficiency is a measure of how effectively a primer set amplifies a target DNA sequence in a polymerase chain reaction (PCR). It is an important parameter in quantitative PCR (qPCR) as it affects the accuracy of the quantification. High primer efficiency indicates that the primers are well-designed and that the PCR conditions are optimal for the amplification of the target sequence.
Let's assume the following value:
Step 1: Use the formula:
\[ E = 10^{\left(-\frac{1}{3.32}\right)} - 1 = 10^{-0.301} - 1 = 0.5 - 1 = -0.5 \times 100 = -50\% \]
The Primer Efficiency is -50%.
Let's assume the following value:
Step 1: Use the formula:
\[ E = 10^{\left(-\frac{1}{3.0}\right)} - 1 = 10^{-0.333} - 1 = 0.464 - 1 = -0.536 \times 100 = -53.6\% \]
The Primer Efficiency is -53.6%.
