To calculate the Molar Ellipticity (\([θ]\)):
\[ [θ] = \frac{θ_{obs} \times MW}{10 \times l \times c} \]
Where:
Molar ellipticity is a measure of the degree to which a substance can rotate plane-polarized light. It is commonly used in the field of circular dichroism spectroscopy to study the chiral properties of molecules, particularly proteins and nucleic acids. The molar ellipticity is calculated from the observed ellipticity, molecular weight, path length, and concentration of the sample. It provides insights into the secondary structure and conformational changes of biomolecules.
Let's assume the following values:
Using the formula:
\[ [θ] = \frac{10 \times 50000}{10 \times 1 \times 0.01} = 5000000 \]
The Molar Ellipticity is 5000000.
Let's assume the following values:
Using the formula:
\[ [θ] = \frac{20 \times 60000}{10 \times 2 \times 0.02} = 3000000 \]
The Molar Ellipticity is 3000000.
