Cell Density Calculator

Formula

To calculate the Cell Density:

\[ CD = \frac{ACSQ \times DF}{VC} \]

Where:

\( CD \) is the cell density per mL
\( ACSQ \) is the average number of cells per square
\( DF \) is the dilution factor (this is 1 if the sample has not been diluted at all)
\( VC \) is the volume of 1 cell (mL)

Cell Density Definition

Cell density is a unit of measure that describes the total number of cells, often only viable living cells, per unit volume. Human tissues average between 1 billion and 3 billion viable living cells per milliliter of tissue.

Example Calculation

Let's assume the following values:

Average Number of Cells per Square \( ACSQ \) = 100
Dilution Factor \( DF \) = 1
Volume of 1 Cell \( VC \) = 0.000001 mL

Step 1: Multiply the average number of cells per square by the dilution factor:

\[ ACSQ \times DF = 100 \times 1 = 100 \]

Step 2: Divide the result by the volume of 1 cell:

\[ CD = \frac{100}{0.000001} = 100,000,000 \text{ cells/mL} \]

The cell density is 100,000,000 cells/mL.