The formula to calculate the Flow Rate (FR) is:
\[ FR = \pi \left( \frac{CD}{10} \right)^2 \times \frac{1}{4} \times LV \]
Where:
The flow rate in high-performance liquid chromatography (HPLC) is the volume of mobile phase (solvent) that passes through the column per unit of time. It is a critical parameter that influences the separation efficiency, analysis time, and resolution of the chromatographic system. The flow rate is determined by the column dimensions and the desired linear velocity of the mobile phase.
Let's say the column diameter (CD) is 4.6 mm and the linear velocity (LV) is 1 cm/min. Using the formula:
\[ FR = \pi \left( \frac{4.6}{10} \right)^2 \times \frac{1}{4} \times 1 \approx 0.166 \text{ mL/min} \]
So, the flow rate (FR) is approximately 0.166 mL/min.
Definition: The column volume calculator helps determine the volume of a chromatography column based on its dimensions.
Formula: \( V = \pi r^2 h \)
Example: \( V = \pi \times 1^2 \times 10 \)
Definition: The vapor volume calculator estimates the volume of vapor produced from a liquid sample.
Formula: \( V_v = \frac{nRT}{P} \)
Example: \( V_v = \frac{1 \times 0.0821 \times 298}{1} \)
Definition: The sample flush out factor determines the volume needed to flush out a sample from a chromatography column.
Formula: \( F = \frac{V_c}{V_s} \)
Example: \( F = \frac{10}{2} \)
Definition: The HPLC method transfer calculator helps in transferring methods between different HPLC systems.
Formula: \( t_R = \frac{L_2}{L_1} \times t_{R1} \)
Example: \( t_R = \frac{150}{100} \times 5 \)
